Dynamin (Dyn1, Hudy 1, DNM1)

Dynamin is a 100kD GTP binding protein that was first identified as a protein able to bind microtubules. A mutation in the Drosophila gene shits, encoding a dynamin homologue, prevents synaptic vesicle recycling and causes temperature-sensitive paralysis, implicating dynamin in the formation of coated vesicles. Dynamin may mediate vesicle formation by assembling into a ring structure around the necks of invaginated coated pits that may eventually lead to the closure of the neck and formation of vesicles. Amphiphysin, dynamin, clathrin and AP2 form a complex during synaptic vesicle endocytosis, which is regulated by the phosphorylated state of different components in this complex. To date, three genes have been found to encode dynamin isoforms. Dynamin 1 is neuronal specific, Dynamin 2 is ubiquitously expressed and Dynamin 3 localizes to testis, brain and lung.\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry: Paraffin sections\nWestern Blot: 0.5mg/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D9900-01C

Size

50ug

Applications

IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Ch/Bird

Form

Supplied as a liquid in PBS, pH 7.2, 0.1mM PMSF, 50% glycerol

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Shpetner, H.S. and Vallee, R.B. (1989) Cell 59: 421-432. 2. van der Bliek, A.M. and Meyerowitz, E.M. (1991) Nature 351: 411-414. 3. Takei, K., McPherson, P.S., Schmid, S.L., and Ce Camilli, P. (1995) Nature 374: 186-190. 4. Slepnev, V.I., Ochoa, G.-C., Butler, M.H., Grabs, D. and De Camilli, P. (1998) Science 281: 821-824.\n5. Cao, H., Garcia, F., McNiven, M.A. (1998) Mol. Biol. Cell. 9: 2595-2609.

Additional Info

Recognizes human Dynamin at ~100kD. Species Crossreactivity: mouse, rat, bovine and chicken.