Dynamin, phosphorylated (Ser774) (Dyn1, Hudy 1, DNM1)

Dynamins are a family of GTPases transcribed from at least three separate genes. They contain several conserved regions; an amino-terminal GTPase domain, a centrally located membrane-binding plekstrin homology domain (PHD), and a region located in front of the proline-rich domain (PRD). This PRD region is thought to mediate interactions between dynamin and other cellular proteins. There are also at least three isoforms of this protein. Dynamin 2 is ubiquitous. Dynamin 3 is expressed in the brain, testis, heart and lung. Dynamin 1 is exclusively neuronal and is phosphorylated by PKC and dephosphorylated by calcineurin during an action potential in the nerve terminal. It is believed that this dephosphorylation provides a trigger for the cellular process of clatherin-mediated and fluid-phase endocytosis.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D9901-20E

Size

100ul

Applications

WB

Hosts

Sheep

Reactivities

Rat, Ch/Bird

Form

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Tan, TC, et al. Cdk5 is Essential for Synaptic Vesicle Endocytosis. Nat. Cell Bio., 5(8): 701-710. 2003

Additional Info

Specific for the ~95k dynamin protein phosphorylated at Ser774. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin). Immunolabeling is blocked by lambda-phosphatase treatment. Species Crossreactivity: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse and non-human primates based on the fact that these species have 100% homology with the amino acid sequence used as antigen.