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Dynamin, phosphorylated (Ser778) (Dyn1, Hudy 1, DNM1)

Dynamin, phosphorylated (Ser778) (Dyn1, Hudy 1, DNM1)

Cat no: D9901-20F

Supplier: United States Biological
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Dynamins are a family of GTPases transcribed from at least three separate genes. They contain several conserved regions; an amino-terminal GTPase domain, a centrally located membrane-binding plekstrin homology domain (PHD), and a region located in front of the proline-rich domain (PRD). This PRD region is thought to mediate interactions between dynamin and other cellular proteins. There are also at least three isoforms of this protein. Dynamin 2 is ubiquitous. Dynamin 3 is expressed in the brain, testis, heart and lung. Dynamin 1 is exclusively neuronal and is phosphorylated by PKC and dephosphorylated by calcineurin during an action potential in the nerve terminal. It is believed that this dephosphorylation provides a trigger for the cellular process of clatherin-mediated and fluid-phase endocytosis. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. The labeling of the Dynamin bands of rat brain is blocked by the phosphopeptide but not by the dephosphopeptide. Optimal dilutions to be determined by the researcher. Cellular Localization: Cytoplasmic Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: D9901-20F
Reactivities: Human, Mouse, Rat
Hosts: Sheep
Applications: Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM Hepes, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Tan, TC, et al. Cdk5 is Essential for Synaptic Vesicle Endocytosis. Nat. Cell Bio., 5(8): 701-710. 2003
Additional info: Recognizes human Dynamin at ~97kD when phosphorylated at Ser778. No crossreactivity was detected when other purified substrates for CDK5 were tested (ie: Synapsin and amphiphysin). Species Crossreactivity: Rat, mouse

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