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Dynein, Cytoplasmic, 74kD Intermediate Chains

Dynein, Cytoplasmic, 74kD Intermediate Chains

Cat no: D9901-50B

Supplier: United States Biological
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The directed movement of membrane-bounded organelles is an important process for various cellular functions including membrane transport, secretion, and axonal transport. Cytoplasmic dynein, a ubiquitous minus end-directed microtubule-based motor protein, is believed to be responsible for the retrograde transport of membranous organelles from the synapse to the cell body in fast axonal transport. The cytoplasmic dynein complex is composed of two 530kD heavy chains, and intermediate chains of ~74kD and 53-59kD. Cellular Localization: Cytoplasmic Applications: Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: D9901-50B
Hosts: Mouse
Applications: Immunofluorescence, Immunoprecipitation, Western Blot
Size: 100ug
Form: PBS containing BSA and 0.05% sodium azide
P type: Mab
Isotype: IgG
Purity: Purified by Protein A affinity chromatography.
References: Journal of Biological Chemistry, 1996; 271: 1687-1694 Journal of Cell Biology, 1992; 118: 1133-1143. Journal of Cell Biology, 1994; 127: 1671-1681 Journal of Cell Science, 2004; 117: 4389-4400. Journal of Virology, Sept. 2004: 10122-10132. Molecular Biology of the Cell, 1996; 7: 331-343. Proceedings of the National Academy of Science, USA, 1996; 93: 141-144
Additional info: Detects two isoforms of the 74kDa polypeptide of cytoplasmic Dynein. Species Crossreactivity: Many

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