Dynein Light Chain 1 (DLC1, LC8, Protein Inhibitor of nNOS, PIN)

DNAL1 was identified as a potential candidate gene for primary ciliary dyskinesia (PCD), a genetically heterologous disorder characterized by chronic infections of the upper and lower airways that often leads to permanent lung damage, randomization of left/right body symmetry, and reduced fertility. DNAL1 is reported to be expressed solely in tissues carrying motile cilia for flagella and interacts with DNAH5, a protein that when mutated has been shown to result in PCD. It has been suggested that DNAL1 serves a regulatory function for DNAH5 activity in outer dynein arms of sperm flagella, respiratory cilia, and ependymal cilia. DNAL1 has also been recently identified as an HIV dependency factor (HDF), suggesting that DNAL1 may be an important drug target in HIV treatment. At least two isoforms of DNAL1 are known to exist.\n\nApplications: \nSuitable for use in Flow Cytometry and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D9901-60

Size

100ug

Applications

FC, WB

Hosts

Rat

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.02% sodium azide.\n

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgM

References

1.Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis: H. Puthalakath, et al.; Science 293, 1829 (2001)

Additional Info

Recognizes mouse DLC1. Does not cross-react with DLC2. For detection of both DLC1 and DLC2 see D9901-65. Species Crossreactivity: human.