Eukaryotic cells rely on actin and microtubule-based protein "motors" to generate intracellular movements. These motor proteins contain specialized domains that hydrolyse ATP to produce force and movement along a cytoskeletal polymer (microtubules in the case of the kinesin family and dyneins) In all cell types, dynein is a large protein complex composed of six distinct subunits and is responsible for most intracellular movement toward the minus ends of microtubules. Cytoplasmic dynein has many important roles in transporting membrane bound organelles, including endosomes, lysosomes, and mitochondria. It is also involved in the positioning of the centrosome, the nucleus, and the Golgi. Cytoplasmic dynein is also responsible for virus transport to the nucleus, retrograde axonal transport, and microtubule and neurofilament transport. Three families of dynein light chains (DYNLT) have been identified. Two isoforms of the DYNLT family of light chains, DYNLT1 and DYNLT3, have been proposed to link dynein to specific cargoes. Recent publications indicate that DYNLT1 binds to Rhodopsin and Doc2 whereas DYNLT3 does not, (W.-Ho, 2007).
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 0.5ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control:
HEK293 cell lysate
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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