DYRK1A (Dual Specificity Tyrosine-phosphorylation-regulated Kinase 1A, Protein Kinase Minibrain Homolog, MNBH, HP86, Dual Specificity YAK1-related Kinase, hMNB, DYRK, MNB, MNBH)

DYRK1A is a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nAA Sequence:\nNQGNQAYQNRPVAANTLDFGQNGAMDVNLTVYSNPRQETGIAGHPTYQFSANTGPAHYMTEGHLTMRQGADREESPMTGVCVQQSPVASS\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

126095

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2b,k

References

1. Mice Deficient in Cystathionine Beta Synthase Display Increased Dyrk1A and SAHH Activities in Brain. Planque C, Dairou J, Noll C, Bui LC, Ripoll C, Guedj F, Delabar JM, Janel N.J Mol Neurosci. 2012 Jun 15. 2. Increased dosage of the chromosome 21 ortholog Dyrk1a promotes megakaryoblastic leukemia in a murine model of Down syndrome. Malinge S, Bliss-Moreau M, Kirsammer G, Diebold L, Chlon T, Gurbuxani S, Crispino JD.J Clin Invest. 2012 Mar 1;122(3):948-62. doi: 10.1172/JCI60455. Epub 2012 Feb 22. 3. DYRK1A: A master regulatory protein controlling brain growth. Guedj F, Pereira PL, Najas S, Barallobre MJ, Chabert C, Souchet B, Sebrie C, Verney C, Herault Y, Arbones M, Delabar JM.Neurobiol Dis. 2012 Jan 26. [Epub ahead of print] 4. Loss of correlations among proteins in brains of the Ts65Dn mouse model of down syndrome. Ahmed MM, Sturgeon X, Ellison M, Davisson MT, Gardiner KJ.J Proteome Res. 2012 Feb 3;11(2):1251-63. Epub 2012 Jan 25. 5. Dyrk1A negatively regulates the actin cytoskeleton through threonine phosphorylation of N-WASP. Park J, Sung JY, Park J, Song WJ, Chang S, Chung KC.J Cell Sci. 2012 Jan 1;125(Pt 1):67-80. Epub 2012 Jan 16. 6. Altered regulation of tau phosphorylation in a mouse model of down syndrome aging. Sheppard O, Plattner F, Rubin A, Slender A, Linehan JM, Brandner S, Tybulewicz VL, Fisher EM, Wiseman FK.Neurobiol Aging. 2011 Aug 13. [Epub ahead of print] 7. Engineering DYRK1A over-dosage yields Down syndrome-characteristic cortical splicing aberrations. Toiber D, Azkona G, Ben-Ari S, Toran N, Soreq H, Dierssen M.Neurobiol Dis. 2010 Jun 30. [Epub ahead of print] 8. DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1. Guo X, Williams JG, Schug TT, Li X.J Biol Chem. 2010 Feb 18. [Epub ahead of print] 9. Hyperhomocysteinemia-induced Dyrk1a downregulation results in cardiomyocyte hypertrophy in rats. Raaf L, Noll C, Cherifi M, Benazzoug Y, Delabar JM, Janel N.Int J Cardiol. 2009 Nov 9. [Epub ahead of print] 10. DYRK1A, a Novel Determinant of the Methionine-Homocysteine Cycle in Different Mouse Models Overexpressing this Down-Syndrome-Associated Kinase Noll C, Planque C, Ripoll C, Guedj F, Diez A, Ducros V, Belin N, Duchon A, Paul JL, Badel A, de Freminville B, Grattau Y, Blehaut H, Herault Y, Janel N, Delabar JM.PLoS One. 2009 Oct 21;4(10):e7540. 11. Negative Feedback Inhibition of NFATc1 by DYRK1A Regulates Bone Homeostasis. Lee Y, Ha J, Kim HJ, Kim YS, Chang EJ, Song WJ, Kim HH.J Biol Chem. 2009 Nov 27;284(48):33343-51. Epub 2009 Oct 2. 12. Nonprimed and DYRK1A-primed GSK3?]-phosphorylation sites on MAP1B regulate microtubule dynamics in growing axons. Scales TM, Lin S, Kraus M, Goold RG, Gordon-Weeks PR.J Cell Sci. 2009 Jul 15;122(Pt 14):2424-35. Epub 2009 Jun 23. 13. Green Tea Polyphenols Rescue of Brain Defects Induced by Overexpression of DYRK1A. Guedj F, Sebrie C, Rivals I, Ledru A, Paly E, Bizot JC, Smith D, Rubin E, Gillet B, Arbones M, Delabar JM.PLoS One. 2009;4(2):e4606. Epub 2009 Feb 26. 14. Effect of hyperhomocysteinemia on the protein kinase DYRK1A in liver of mice. Hamelet J, Noll C, Ripoll C, Paul JL, Janel N, Delabar JM.Biochem Biophys Res Commun. 2009 Jan 16;378(3):673-7. Epub 2008 Dec 6. 15. Sprouty2 inhibitory activity on FGF-signaling is modulated by the protein kinase DYRK1A. Aranda S, Alvarez M, Turro S, Laguna A, de la Luna S.Mol Cell Biol. 2008 Oct;28(19):5899-911. Epub 2008 Aug 4.

Additional Info

Recognizes human DYRK1A. Species Crossreactivity: mouse and rat.