DYRK1B, CT (Dual Specificity Tyrosine-phosphorylation-regulated Kinase 1B, Minibrain-related Kinase, Mirk Protein Kinase, MIRK)

The DYRK family includes several dual-specificity tyrosine-phosphorylated and regulated kinases capable of phosphorylating proteins at both tyrosine and serine/threonine residues (1). The DYRK family was identified based on homology to the yeast Yak1 (2) and the Drosophila minibrain (mnb) kinases (3). Seven mammalian isoforms have been discovered, including DYRK1A, DYRK1B, DYRK1C, DYRK2, DYRK3, DYRK4, and DYRK4B. Differences in substrate specificity, expression, and subcellular localization are seen across the DYRK family (4,5). All DYRK proteins have a Tyr-X-Tyr motif in the catalytic domain activation loop, with phosphorylation of the second Tyr residue (e.g. Tyr312 of DYRK1A) necessary for kinase activity. DYRKs typically autophosphorylate the tyrosine residue within their activation loop, but phosphorylate substrates at serine and threonine residues (1,6). In contrast to the ubiquitous DYRK1A, DYRK1B exhibits relatively restricted expression with highest levels found in the testis and muscle (7,8). Three major DYRK1B splice variants demonstrate distinct expression patterns and functional properties (9). DYRK1B plays a critical role in myoblast differentiation by affecting cell motility, transcription, cell cycle progression, and survival (10,11). In addition, DYRK1B contributes to the survival of certain cancer cells (7,12,13). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.