DYRK2 (Dual-specificity tyrosine [Y] phosphorylation regulated kinase 2) is a 58-62kD member of the MNB/DYRK subfamily, CMGC Ser/Thr protein kinase family of enzymes. It is expressed in testis and shows dual substrate specificity; autophosphorylation on Tyr382 to self-activate, and a Ser/Thr phosphorylation of target molecules. Substrates include NFATc, glycogen synthase, and p53. p53 phosphorylation on Ser46 initiates cell apoptosis. Human DYRK2 is 601aa in length. It contains one kinase catalytic domain (aa222-535). One isoform variant shows an alternate start site at Met74. This shorter isoform was used for immunization. Thus, over aa1-107 (or aa74 - 180 of the long form), human DYRK2 shares 93% aa identity with mouse DYRK.
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1ug/ml
Immunohistochemistry: 25ug/ml
Optimal dilutions to be determined by the researcher.
Hybridoma:
Myeloma cells with B cells from Balb/c mice.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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