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E Epitope Tag (GAPVPYPDPLEPR)

E Epitope Tag (GAPVPYPDPLEPR)

Cat no: E0004-01K

Supplier: United States Biological
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The recombinant protein over-expression systems with well-known epitope tags are powerful strategies in checking and purifying the interest proteins, which are expressed in certain vectors containing the particular tagged fragments. The small tags are used to be cloned along with the target proteins, and the antibodies against those fusion-tags are used to monitor fusion protein expression and purification. E-Tag (GAPVPYPDPLEPR) is a popular epitope tag incorporated in the amino or carboxy terminus of the target protein. This antibody can be used to check E- tagged fusion proteins. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: E0004-01K
Hosts: Rabbit
Applications: Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Binz HK et al (2004) J. Nat. Biotechnol. 22(5), 575-582 2. Kolodziej, PA and Young RA. (1991) Methods Enzymol., 194:508-19. 3. Lu Q et al (1997) J. Gene 200(1-2), 135-144. 4. Malby RL et al (1993) J. Proteins 16(1), 57-63. 5. Wilson, I. A., Niman, H. L., Houghten, R. A., Cherenson, A. R., Connolly, M. L., and Lerner, R. A. (1984) Cell 37, 767-778.
Additional info: Recognizes E-Tag in all species.

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