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E2A (E2A Immunoglobulin Enhancer Binding Factors E12/E47, Immunoglobulin Transcription Factor 1, ITF1, Transcription Factor 3, TCF3)

Cat no: E0006-05

E2A (E2A Immunoglobulin Enhancer Binding Factors E12/E47, Immunoglobulin Transcription Factor 1, ITF1, Transcription Factor 3, TCF3)

E2A or Transcription factor 3 (Tcf3), a 68 kD molecule, plays major roles in determining tissue-specific cell fate during embryogenesis. E2A/Tcf3 is involved in a form of pre-B-cell acute lymphoblastic leukemia through a chromosomal translocation t(1;19)(q23;p13.3), which involves pbx1 and TCF3. E2A/Tcf3 is widely expressed and located in the nucleus. Three of the four Lef/TCF genes of the mouse have been shown to function by switching on the Wnt signaling pathway.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nELISA: 0.1-1ug/ml\nWestern Blot: 1-3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n

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SPECIFICATIONS

Catalog Number

E0006-05

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, containing 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A chromatography

Isotype

IgG2b,k

References

1. Merrill B et al, (2004) Development 131(2):263-74.

Additional Info

Recognizes human E2A/Tcf3 at ~68kDa. Reactivity has been confirmed with human Raji cells. Species sequence homology: mouse, rat 88%.

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