E2F3 Transcription Factor

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner.\nE2f transcription factors are functionally regulated by binding to RB p110, p107, and p130. \n\nApplications: \nSuitable for use in Gel Supershift and Immunofluorescence. Other applications not tested.\n\nRecommended Dilution:\nGel Supershift: 1mg/ml\nOptimal dilutions to be determined by the researcher.\n\nRecommended Positive Control:\nRaji Cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. \n

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

E0013-10

Size

250ul

Applications

IF

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See E0013-10X.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

References

1. Adams, P. D., and W. G. Kaelin Jr., (1996) P. J. Farnham 208:79-95. 2. Asken, D. S., et al., (1991) Oncogene. 6:1915-1922. 3. Bandara, L. R., et al., (1993) EMBO J. 12:4317-4324. 4. Beijersbergen, R. L., et al., (1994) Genes & Dev. 8:2680-2690. 5. Buck, V., et al., (1995) Oncogene. 11:31-38. 6. Cao, L., et al., (1992) Nature 355:176-179. 7. Chittenden, T., et al., (1993) Mol. Cell. Biol. 13:3975-3983. 8. Cobrinik, D., et al., (1993) Genes & Develop. 7:2392-2404. 9. Corbeil, H. B., P. Whyte, and P. E. Branton, (1995) Oncogene. 11:909-920. 10. Cress, W. D., and J. R. Nevins, (1993) Mol. Cell. Biol. 13:6314-6325.

Additional Info

Recognizes human E2F-3.