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E2F4 Transcription Factor

Cat no: E0014

E2F4 Transcription Factor

E2f transcription factors are functionally regulated by binding to RB p110, p107, and p130. \n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml detected E2F4 in RIPA lysates from non-stimulated A431 cell lysate. Previous lots have detected E2F4 in HeLa nuclear extract, mouse 3T3 fibroblasts, and rat L6 skeletal myoblasts.\nImmunoprecipitation: 4-10ug immunoprecipitated E2F4 from 500ug of mouse 3T3/A431 cell lysate\n\nOptimal dilutions to be determined by the researcher.\n\nPostive Control:\nA0001-50 included.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E0014

Size

200ug

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as liquid in 0.1M Tris-glycine buffer, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

References

1. Ginsberg, D., et. al., (1994) Genes Develop. 8:2665

Additional Info

Recognizes E2F-4 at 55kD. Species Crossreactivity: Human, mouse and rat.

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