E2F4 (Transcription Factor E2F4, E2F-4)

E2F4 is the most common E2F family member in a number of cell types. E2F4 deficiency has been shown to cause a decrease in the thickness of the gut epithelium, decrease in maturation of hematopoietic cell types and an increased rate of apoptosis, suggesting that E2F4 plays a role in normal maturation of several cell types. E2F4 has also been shown to be essential for the normal development of airway epithelium and early eye development. Other data has implicated E2F4 during normal heart development, specifically cardiomyocyte proliferation.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nHeLa lyate cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

029459

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Van Amerongen, M.J., et al. (2010). Cardiovasc Res. 86(1):92-102. 2. Ruzhynsky, V.A., et al. (2009). Dev Neurosci. 31(3):238-246. 3. Danielian, P.S., et al. (2007). Dev Biol. 305(2): 564-576. 4. Kinross, K., et al. (2006). Blood. 108(3):886-895. 5. Rempel, R.E., et al. (2000). Mol Cell. 6(2):293-306.

Additional Info

Recognizes human E2F4.