Home  >  Products  >  EBAG9, Recombinant, Human (Estrogen Receptor-binding Fragment-associated Gene 9 Protein, EB9, Cancer-associated Surface Antigen RCAS1, PDAF, Receptor-binding Cancer Antigen Expressed on SiSo Cells, RCAS1)
EBAG9, Recombinant, Human (Estrogen Receptor-binding Fragment-associated Gene 9 Protein, EB9, Cancer-associated Surface Antigen RCAS1, PDAF, Receptor-binding Cancer Antigen Expressed on SiSo Cells, RCAS1)

EBAG9, Recombinant, Human (Estrogen Receptor-binding Fragment-associated Gene 9 Protein, EB9, Cancer-associated Surface Antigen RCAS1, PDAF, Receptor-binding Cancer Antigen Expressed on SiSo Cells, RCAS1)

Cat no: E0255-55B

Supplier: United States Biological
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Estrogen receptor binding site associated antigen 9, also known as EBAG9, is a type III transmembrane protein that is predominantly located in the Golgi. This protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers, such as advanced breast and prostate cancers. As the EBAG9 acts to inhibit the growth of receptor-binding cells and induced apoptosis of immune cells, cancer cells might evade immune surveillance. So immunodetection of EBAG9 expression can be a negative prognostic indicator. Recombinant human EBAG9 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. Source: Recombinant corresponding to aa28-213 of human EBAG9 (His-tagged) expressed in E. coli. AA Sequence: MGSSHHHHHH SSGLVPRGSH MRSGRGRKLS GDQITLPTTV DYSSVPKQTD VEEWTSWDED APTSVKIEGG NGNVATQQNS LEQLEPDYFK DMTPTIRKTQ KIVIKKREPL NFGIPDGSTG FSSRLAATQD LPFIHQSSEL GDLDTWQENT NAWEEEEDAA WQAEEVLRQQ KLADREKRAA EQQRKKMEKE AQRLMKKEQN KIGVKLS Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 3ug/ml 15% SDS-PAGE, ~23.4kD Optimal dilutions to be determined by the researcher. Storage and Stability: Can be stored at +4 degrees C short term (1-2 weeks). For long term storage, aliquot and store at -20 degrees C or -70 degrees C. Avoid repeated freezing and thawing cycles.
Catalogue number: E0255-55B
Size: 100ug
Form: Supplied as a liquid in 20mM Tris pH8.0,1mM DTT, 2mM EDTA, 10% glycerol.
Purity: Purified (same/more than) 90% by SDS PAGE.
Alternative names: Estrogen Receptor Binding Site Associated, Antigen, 9, HGNC:3123
References: 1. Kumagai J., et al.,(2009) Int J Cancer.124(4):816-26. 2. Yoshida S., et al.,(2008) J Rheumatol. 35(9):1716-22.

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