EBF1 (COE1, Early B-cell factor 1, COE1, EBF, O/E-1, OLF1, Collier, Olf and EBF transcription factor 1, early B-cell factor olfactory neuronal transcription factor 1)

EBF1 belongs to the COE family. It contains 1 IPT/TIG domain. EBF1 is a transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Forms either a homodimer or a heterodimer with a related family member. Interacts with ZNF423 and ZNF521, leading to prevent EBF1 to bind DNA and activate target genes.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nPeptide ELISA: 1:64,000\nWestern Blot: 0.3-0.1ug/ml detects a band at ~75kD observed in human Adipose lysates (calculatd MW: 64.5kD according to NP_076870.1) \nImmunoprecipitation: reported to immunoprecipitate EBF1 from induced 3T3-L1 expressing FLAG-EBF1\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nHuman Adipose lysates\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E0999-05

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris-saline, pH 7.3, 0.5% BSA, 0.02% sodium azide, 0.5% BSA.

P Type

Pab

Purity

Purified by peptide affinity chromatography.

Isotype

IgG

References

1. Lagergren A, Mansson R, Zetterblad J, Smith E, Basta B, Bryder D, Akerblad P, Sigvardsson M.\nThe CXCL12, periostin and CCL9 genes are direct targets for early B-cell factor (EBF) in OP-9 stroma cells.\nJ Biol Chem. 2007 Mar 20; [Epub ahead of print].PMID: 17374609

Additional Info

Recognizes human EBF1 at ~75kD. Does not cross-react with EBF2, EBF3 and EBF4. Species sequence homology: mouse, rat.