Home  >  Products  >  EBF1 (COE1, Early B-cell factor 1, COE1, EBF, O/E-1, OLF1, Collier, Olf and EBF transcription factor 1, early B-cell factor olfactory neuronal transcription factor 1)
EBF1 (COE1, Early B-cell factor 1, COE1, EBF, O/E-1, OLF1, Collier, Olf and EBF transcription factor 1, early B-cell factor olfactory neuronal transcription factor 1)

EBF1 (COE1, Early B-cell factor 1, COE1, EBF, O/E-1, OLF1, Collier, Olf and EBF transcription factor 1, early B-cell factor olfactory neuronal transcription factor 1)

Cat no: E0999-05

Supplier: United States Biological
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EBF1 belongs to the COE family. It contains 1 IPT/TIG domain. EBF1 is a transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Forms either a homodimer or a heterodimer with a related family member. Interacts with ZNF423 and ZNF521, leading to prevent EBF1 to bind DNA and activate target genes. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:64,000 Western Blot: 0.3-0.1ug/ml detects a band at ~75kD observed in human Adipose lysates (calculatd MW: 64.5kD according to NP_076870.1) Immunoprecipitation: reported to immunoprecipitate EBF1 from induced 3T3-L1 expressing FLAG-EBF1 Optimal dilutions to be determined by the researcher. Positive Control: Human Adipose lysates Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: E0999-05
Reactivities: Human
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris-saline, pH 7.3, 0.5% BSA, 0.02% sodium azide, 0.5% BSA.
P type: Pab
Isotype: IgG
Purity: Purified by peptide affinity chromatography.
References: 1. Lagergren A, Mansson R, Zetterblad J, Smith E, Basta B, Bryder D, Akerblad P, Sigvardsson M. The CXCL12, periostin and CCL9 genes are direct targets for early B-cell factor (EBF) in OP-9 stroma cells. J Biol Chem. 2007 Mar 20; [Epub ahead of print].PMID: 17374609
Additional info: Recognizes human EBF1 at ~75kD. Does not cross-react with EBF2, EBF3 and EBF4. Species sequence homology: mouse, rat.

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