EBP50 (ERM (Ezrin-Radixin-Moesin) Binding Phosphoprotein of 50kD, Na(+)/H(+) Exchange Regulatory Cofactor NHE-RF1, NHERF, NHERF1, NHERF-1, NPHLOP2, Regulatory Cofactor of Na(+)/H(+) Exchanger, Sodium Hydrogen Exchanger Regulatory Factor 1, Solute Carrier

EBP 50 [ERM (ezrin-radixin-moesin) binding phosphoprotein of 50kD] is a PDZ containing protein that is involved in the linkage of integral membrane proteins to the cytoskeleton. EBP50 contains two tandem PDZ domains followed by a carboxy-terminal sequence that binds to members of the ERM family of membrane-cytoskeleton adaptors. The PDZ domains within EBP50 bind to the carboxy termini of such target proteins as beta2 adrenergic receptor, platelet-derived growth factor receptor (PDGFR), and the cystic fibrosis conductance regulator (CFTR). The PDZ domains are also believed to be involved in the oligomerization of EBP 50 to form multiprotein complexes which helps to facilitate the formation of functional signalling complexes. EBP 50 has been shown to link integral membrane proteins to the cytoskeleton by binding to members of the ERM family of proteins, which bind to F-actin. Through these interactions, EBP 50 is implicated in the localization of interactive groups of proteins into subcellular domains and in the regulation of activity of those interacting proteins. Immunohistochemical studies have shown that EBP 50 is found exclusively at the apical membranes of epithelial cells. \n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. \n\nRecommended Dilutions:\nWestern Blot: 1ug/ml \nImmunohistochemistry: Staining of EBP50 in mouse airway results in staining of the epithelium.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E1050

Size

100ug

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide. Also available with Biotin and without BSA or azide. See E1050B.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1.Fouassier L, et al., (2001) Hepatology 33:166-76. 2.Fouassier L, et al., (2000) J Biol Chem 275:25039-45. 3. Bretscher A, et al., (2000) Annu Rev Cell Dev Biol 16:113-43.

Additional Info

Recognizes EBP 50 from human T84 and Calu3 cells as well as recombinant human protein at ~50kD. Species Crossreactivity: mouse.