EBP50 (ERM (Ezrin-Radixin-Moesin) Binding Phosphoprotein of 50kD, Na(+)/H(+) Exchange Regulatory Cofactor NHE-RF1, NHERF, NHERF1, NHERF-1, NPHLOP2, Regulatory Cofactor of Na(+)/H(+) Exchanger, Sodium Hydrogen Exchanger Regulatory Factor 1, Solute Carrier

EBP 50 [ERM (ezrin-radixin-moesin) binding phosphoprotein of 50kD] is a PDZ containing protein that is involved in the linkage of integral membrane proteins to the cytoskeleton. EBP50 contains two tandem PDZ domains followed by a carboxy-terminal sequence that binds to members of the ERM family of membrane-cytoskeleton adaptors. The PDZ domains within EBP50 bind to the carboxy termini of such target proteins as beta2 adrenergic receptor, platelet-derived growth factor receptor (PDGFR), and the cystic fibrosis conductance regulator (CFTR). The PDZ domains are also believed to be involved in the oligomerization of EBP 50 to form multiprotein complexes which helps to facilitate the formation of functional signalling complexes. \n\nEBP 50 has been shown to link integral membrane proteins to the cytoskeleton by binding to members of the ERM family of proteins, which bind to F-actin. Through these interactions, EBP 50 is implicated in the localization of interactive groups of proteins into subcellular domains and in the regulation of activity of those interacting proteins. Immunohistochemical studies have shown that EBP 50 is found exclusively at the apical membranes of epithelial cells. \n\nApplication(s):\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested. \n\nRecommended Dilution:\nWestern Blot: 1ug/ml \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E1050B

Size

50ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Conjugates

Biotin

Form

Supplied as a liquid in PBS. Conjugated to Biotin. No preservative added. No stabilizing proteins added. Also available without Biotin. See E1050.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Fouassier L et al. (2001) Hepatology 33:166-76. 2. Fouassier L et al. (2000) J Biol Chem 275:25039-45. 3. Bretscher A et al. (2000) Annu Rev Cell Dev Biol 16:113-43.

Additional Info

Recognizes EBP 50 from human T84 and Calu3 cells as well as recombinant human protein at ~50kD. Stains the epithelium of mouse airway in Immunohistochemistry. Species Crossreactivity: Mouse.