EBP50 (ERM (Ezrin-Radixin-Moesin) Binding Phosphoprotein of 50kD, Na(+)/H(+) Exchange Regulatory Cofactor NHE-RF1, NHERF, NHERF1, NHERF-1, NPHLOP2, Regulatory Cofactor of Na(+)/H(+) Exchanger, Sodium Hydrogen Exchanger Regulatory Factor 1, Solute Carrier

EBP 50 [ERM (ezrin-radixin-moesin) binding phosphoprotein of 50kD] is a PDZ containing protein that is involved in the linkage of integral membrane proteins to the cytoskeleton. EBP50 contains two tandem PDZ domains followed by a carboxy-terminal sequence that binds to members of the ERM family of membrane-cytoskeleton adaptors. The PDZ domains within EBP50 bind to the carboxy termini of such target proteins as beta2 adrenergic receptor, platelet-derived growth factor receptor (PDGFR), and the cystic fibrosis conductance regulator (CFTR). The PDZ domains are also believed to be involved in the oligomerization of EBP 50 to form multiprotein complexes which helps to facilitate the formation of functional signalling complexes. \n\nEBP 50 has been shown to link integral membrane proteins to the cytoskeleton by binding to members of the ERM family of proteins, which bind to F-actin. Through these interactions, EBP 50 is implicated in the localization of interactive groups of proteins into subcellular domains and in the regulation of activity of those interacting proteins. Immunohistochemical studies have shown that EBP 50 is found exclusively at the apical membranes of epithelial cells. \n\nApplications: \nSuitable for use in Western Blot, Immunocytochemistry, and Immunofluorescence. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1,000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunocytochemistry/Immunofluorescence: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E1050C

Size

100ul

Applications

ICC, IF, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

P Type

Pab

Purity

Purified by peptide affinity chromatography.

Isotype

IgG

References

1. Donowitz, M. et al. (2005) J Physiol 567, 3-11. 2. Voltz, J.W. et al. (2001) Oncogene 20, 6309-14. 3. Stemmer-Rachamimov, A.O. et al. (2001) Am J Pathol 158, 57-62. 4. Takahashi, Y. et al. (2006) EMBO J 25, 910-20. 5. Wheeler, D. et al. (2007) J Biol Chem 282, 25076-87. 6.Weinman, E.J. et al. (2000) Am J Physiol Renal Physiol 279, F393-9.

Additional Info

Detects endogenous levels of human total EBP50 protein.