Echinococcus, Human, BioAssay(TM) ELISA Kit

For the qualitative screening of serum IgG antibodies to Echinococcus sp. using an ELISA technique.\n\nEchinococcosis (hydatidosis) is the infection caused by cestodes of the genus Echinococcus. Humans are potential intermediate hosts and can become infected by ingesting eggs passed in the feces of an infected animal. The resulting disease is called hydatidosis, or hydatid disease. Four species are known pathogens of the disease, E. granulosus, E. multilocularis, E. oligarthrus and E. vogeli. The infection caused by E. granulosus is referred to as cystic hydatid disease (CHD) and results in cysts in various organs, especially the liver and lungs. These cysts may become quite large and contain hundreds or thousands of scoleces called hydatid sand. The degree of antibody response to these cysts will vary depending on their location and degree of calcification. Liver cysts typically produce a higher antibody response than lung cysts. Infection due to E. multilocularis is referred to as alveolar hydatid disease (AHD), and also occurs as cysts that may spread throughout the infected tissue.\n\nSince Echinococcus eggs are not shed by infected humans, serological determination has been important in the diagnosis of hydatid disease. A number of tests have been used, including latex agglutination (LA), indirect hemagglutination (IHA), complement fixation (CF), agar gel diffusion (AGD) and enzyme linked immunosorbent assay (ELISA). Cross reactivity between echinococcosis and cysticercosis (Taenia solium infection) will occur to some degree in this assay due to the use of crude antigen. It is recommended that any sample showing a positive result by this test be confirmed by additional testing. \n\nKit Components:\n1. Microtiter Plate (Echinococcus antigens): 1x96wells\n2. Protein A (HRP): 1x11ml \n3. Positive Control: 1x2ml \n4. Negative Control: 1x2ml \n5. Tetramethylbenzidine (TMB): 1x11ml\n6. Wash Buffer, 20X: 1x25ml\n7. Dilution Buffer: 2x30ml \n8. Stop Solution: 1x11ml\n \nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E0257

Size

1Kit

Applications

ELISA

Reactivities

Hum

References

1. Schantz, P. and B. Gottstein. \"Echinococcosis (Hydatidosis).\" Immunodiagnosis of Parasitic Diseases, Vol. 1, Helminthic Diseases. Ed. Walls and Schantz. Academic Press, 1986. pp. 69-107 2. Krogstad D., G. Visvesvara R. Walls, J. Smith. \"Tissue Helminths.\" Manual of Clinical Microbiology, 4th Ed.. American Society for Microbiology, Washington, DC. 1985, pp. 657-659 3. Kagan, I. \"Serodiagnosis of Parasitic Diseases.\" Manual of Clinical Laboratory Immunology. 3rd Ed.. American Society for Microbiology, Washington, DC. 1986, p. 472 4. Ash, L. and T. Orihel. \"Parasites - A Guide to Laboratory Procedures and Identification.\" 1987, ASCP Press, pp. 176-177 5. Coltorti, E. \"Standardization and Evaluation of an Enzyme Immunoassay as a Screening Test for the Seroepidemiology of Human Hydatidosis.\" Am J Trop Med Hyg 35:5, 1986, pp. 1000-1005 6. Coltorti, E. et. al. \"Field Evaluation of an Enzyme Immunoassay for Detection of Asymptomatic Patients in a Hydatid Control Program.\" Am J Trop Med Hyg, 38: 3, 1988, pp. 603-607.