ECL Cell Attachment Matrix (Entactin-Collagen IV-Laminin)

Supplier:

United States Biological

Cat no: E0275

ECL Cell Attachment Matrix (Entactin-Collagen IV-Laminin)

Neurite Outgrowth Assay:\nDilute E0275 with sterile serum-free medium to ~20ug/ml. Add directly to culture vessels to achieve 5-10ug/cm2.\nAllow the matrix proteins to adsorb to the vessel for 1 hour at 37C or overnight at 4C prior to adding to a cell.\n\nECL was tested at 5ug/cm2 and found to promote neurite outgrowth using PC12 rat pheochromocytoma cells.\n\nNote: Color variations from yellow to dark red may occur in frozen or thawed vials of ECL These color variations are caused by the interaction of CO2 with the buffer and phenol red present in the product. Such color variations are normal and do not affect the efficacy of the product. \n\nSterility: \nNegative for the presence of bacteria, fungi and mycoplasma. \n\nStorage: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E0275

Size

5mg

Form

Supplied as a liquid in 0.05M Tris-HCl, pH 7.4, 0.15M sodium chloride.

Purity

Protein determined by the Bradford dye binding assay using gamma globulin as the standard.

References

1. Kleinman, H.K., et al., (1986) Biochemistry 25:312. 2. Kleinman, H.K., et al., (1982) Biochemistry 21:6188. 3. Hadley, M.A., et al., (1985) J. Cell. Biol. 101:1511.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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