EcoRI 10u/ul

Supplier:

United States Biological

Cat no: E0375

EcoRI 10u/ul

5'-G^A A T T C-3' \n3'-C T T A A^G-5'\n\nSupplied With:\nR1625: Restriction Enzyme Buffer A, 10X\nR1625-03: Restriction Enzyme Buffer D, 10X \n\nUnit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37 degrees C in 50ul of assay buffer.\n\nIncubation Temperature: 37 degrees C\n\nDilution:\nFor short-term storage (3-4 weeks):\nDilute with Diluent Buffer (R1625-05, 10mM Tris-HCl, pH 7.4 at 25 degrees C, 100mM potassium chloride, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.\n\nFor long-term storage:\nDilute with Storage Buffer (10mM potassium phosphate, pH 7.4, 25 degrees C, 300mM NaCl, 1mM EDTA, 7mM 2-mercaptoethanol, 0.2mg/ml BSA, 0.15% Triton X-100, 50% glycerol).\n\nQuality Control:\nOverdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion (5u/ug lambda DNA x 16 hours) with EcoRI.\n\nLigation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with EcoRI, more than 90% of the DNA fragments can be ligated at a 5'-termini concentration of 0.02uM. More than 90% of these can be recut. \n\nLabeled Oligonucleotide Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10u of restriction endonuclease for 4 hours.\n\nBlue/White Cloning Assay: pUC57 was digested at a unique site with 10u of enzyme for 16 hours. After religation and transformation 0.2% of white colonies were detected.\n\n\nMethylation Effects: EcoRI cuts neither Gm6AATTC nor GAm6ATTC, but cuts GAATTm5C, GAATThm5C and GAAhm5Uhm5UC.\n\nStorage and Stability:\nStore at -20C. Stable for 12-12 months.

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SPECIFICATIONS

Catalog Number

E0375

Size

5x5000u

Read more on Supplier website

SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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