The ecto-nucleoside triphosphate diphosphohydrolases (eNTPDases) are a family of enzymes that control extracellular nucleotide levels, thereby regulating purinergically controlled physiological processes. Six of the eight known NTPDases are membrane-bound, and only ENTPD5 and ENTPD6 can be released as soluble enzymes (1). ENTPD5 promotes reglycosylation reactions involved in glycoproteins folding and quality control in the endoplasmic reticulum. It also hydrolyzes UDP, GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate (2). ENTPD5 is expressed in adult liver, kidney, prostate, testis and colon (1).
Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Western Blot: 1:1000-1:10,000
Immunohistochemistry (Paraffin): 1:100-1:250
Immunoprecipitation: 1:10-1:100
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.