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Ectonucleotide Pyrophosphatase, Phosphodiesterase 7 (ENPP-7, Gm254, Alk-SMase, Enpp7)

Cat no: E3224-08

Ectonucleotide Pyrophosphatase, Phosphodiesterase 7 (ENPP-7, Gm254, Alk-SMase, Enpp7)

SENPP-7 (ectonucleotide pyrophosphatase/phosphodiesterase 7), also known as alkaline sphingomyelinase (Alk-SMase)is a 60kD GPI-linked membrane glycoprotein expressed in the intestines and in human bile. It shares 30%-36% homology with the NPP family, but is unrelated to neutral or acid SMase. ENPP-7 hydrolyzes dietary sphingomyelin to form ceramide and phosphorylcholine, and may also hydrolyze and inactivate platelet-activating factor (PAF). It is downregulated in some human colorectal carcinomas. The 416aa mature human ENPP-7 shares 80% and 82% aa identity with mouse and rat ENPP-7, respectively.\n\nApplications: \nSuitable for use in ELISA, Western Blot. Other applications not tested.\n\nRecommended Dilution:\nDirect ELISA: 0.5-1ug/ml \nWestern Blot: 2ug/ml \nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nMyeloma cells with B cells from Balb/c mice. \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3224-08

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with sterile PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

Additional Info

Recognizes human ENPP-7.

Read more on Supplier website

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

More info
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