SENPP-7 (ectonucleotide pyrophosphatase/phosphodiesterase 7), also known as alkaline sphingomyelinase (Alk-SMase)is a 60kD GPI-linked membrane glycoprotein expressed in the intestines and in human bile. It shares 30%-36% homology with the NPP family, but is unrelated to neutral or acid SMase. ENPP-7 hydrolyzes dietary sphingomyelin to form ceramide and phosphorylcholine, and may also hydrolyze and inactivate platelet-activating factor (PAF). It is downregulated in some human colorectal carcinomas. The 416aa mature human ENPP-7 shares 80% and 82% aa identity with mouse and rat ENPP-7, respectively.
Applications:
Suitable for use in ELISA, Western Blot. Other applications not tested.
Recommended Dilution:
Direct ELISA: 0.5-1ug/ml
Western Blot: 2ug/ml
Optimal dilutions to be determined by the researcher.
Hybridoma:
Myeloma cells with B cells from Balb/c mice.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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