EDG 3 (Endothelial Cell Differentiation Gene 63, Sphingosine-1-phosphate Receptor 3, S1PR3)

Sphingosine-1-phosphate (S1P) exerts its activity by binding to five distinct G-protein-coupled receptors, S1P1/EDG-1, S1P2/EDG-5, S1P3/EDG-3, S1P4/EDG-6, and S1P5/EDG-8. S1P3 couples to Gi/o-ERK, Gq-PLC, and G12/13-Rho axes to mediate S1P-induced cell proliferation, survival, migration, and related signaling events. S1P3 is widely expressed in various tissues, suggesting diverse physiological functions of this receptor. The human and murine S1P3 have 378 amino acids with an estimated molecular weight of 42kD. Glycosylation at the N-terminal extracellular domain may cause the protein to migrate at different positions in SDS-PAGE. \n\nApplications: \nSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:200\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E2208-15B

Size

100ug

Applications

ICC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in TBS, pH 7.4, 5mg/ml BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Takuwa, Y., et al., (2002) J. Biochem. 131:767-771. 2. Ishii, I., et al., (2004) Annu. Rev. Biochem. 73:321-354 . 3. Kluk, M.J. & Hla, T., (2002) Biochim. Biophys. Acta 1582:72-80. 4. Takuwa, Y., (2002) Biochem. Biophys. Acta 1582:112-120.

Additional Info

Recognizes human EDG 3 receptor at ~47kD in human cerebral cortex. Liver and heart tissues display 2-3 bands between 40-50kD, possibly due to different degrees of glycosylation. Species Crossreactivity: mouse and rat.