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EDG 6 (Endothelial Differentiation G-protein Coupled Receptor 6, EDG6, LPC1, SLP4, Sphingosine 1-phosphate Receptor 4, S1P Receptor 4, S1P4, S1PR4, Sphingosine 1-phosphate Receptor Edg-6, S1P Receptor Edg-6)

Cat no: E2208-30D

EDG 6 (Endothelial Differentiation G-protein Coupled Receptor 6, EDG6, LPC1, SLP4, Sphingosine 1-phosphate Receptor 4, S1P Receptor 4, S1P4, S1PR4, Sphingosine 1-phosphate Receptor Edg-6, S1P Receptor Edg-6)

Edg6 is a recently cloned member of the endothelial differentiation gene (EDG) G protein-coupled receptor family. It is a receptor for the lysosphingolipid sphingosine 1-phosphate (S1P), which are structurally related lysophospholipid mediators. Binding of SPP to Edg6 activates Erk, leading to the activation of the transcription factor Elk1 and thus the mitogen-activated protein kinase (MAPK) signal transduction pathway. The protein also acts as a receptor for a lipid-derived ligand. Edg6 plays an essential role in lymphocyte cell signaling. The protein is specifically expressed in fetal and adult lymphoid and hematopoietic tissue as well as in lung. Human Edg6 gene is intronless and is mapped to chromosome 19p13.3 at the D19S120 marker.\n\nApplications:\nSuitable for use in Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nImmunofluorescence (IC): 5ug/ml\nImmunohistochemistry: Paraffin sections\nWestern Blot: 3-5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nJurkat and RAW cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E2208-30D

Size

100ug

Applications

IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Markus, HG. et al. Genomics. 53:164-169 (1998). 2. Jaillard, C. et al. The Journal of Neuroscience. 25:1459-1469 (2005).

Additional Info

Recognizes human Sphingolipid Receptor Edg6/S1P4. \nSpecies Crossreactivity: mouse

Alternative Names

Endothelial Cell Differentiation Gene 6

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