EDG1 is a member of the endothelial differentiation gene family. It is a high affinity G protein-coupled receptor for the bioactive lipid, Sphingosine-1-phosphate. EDG1 signaling regulates endothelial cell survival, cytoskeletal remodeling, chemotaxis and angiogenesis.
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilutions:
Flow Cytometry: 25ug/ml. 10ul labels 1-2.5x10e5 cells in a total reaction volume (same/less than)200ul.
Optimal dilutions to be determined by the researcher.
Hybridoma:
Myeloma cells with spleen cells from Balb/c mice with NS0-derived, recombinant human Endothelial Differentiation Gene 1 (hEDG1).
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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