EDG1 (Endothelial Cell Differentiation Gene 1) (PE)

EDG1 is a member of the endothelial differentiation gene family. It is a high affinity G protein-coupled receptor for the bioactive lipid, Sphingosine-1-phosphate. EDG-1 signaling regulates endothelial cell survival, cytoskeletal remodeling, chemotaxis and angiogenesis.\n\nApplications: \nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: Neat. 10ul labels 10e6 cells. Validated using human EDG1 transfected CHO-S cells. Cell surface expression is determined using 488nm wavelength excitation and monitoring emitted fluorescence with a detector optimized to collect peak emissions at 565-605nm.\nOptimal dilutions to be determined by the researcher.\n\nFlow Cytometry Protocol:\n1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.\n2. Add 10ul of E2208-01E to (same/less than)1x10e6 cells and incubate for 30 minutes at RT.\n3. Removed unbound antibody by washing the cells twice with Flow Cytometry Staining Buffer (F4568-10). Note that whole blood requires a RBC lysis step at this point. \n4. Resuspend the cells in F4568-10 for analysis. As a control, cells in a separate tube should be treated with PE-labeled mouse IgG2b antibody. \n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C. Stable for 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

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