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EED (Embryonic ectoderm development, hEED, HEED, Polycomb protein EED, WAIT1, WAIT-1, WD protein associating with integrin cytoplasmic tails 1)

EED (Embryonic ectoderm development, hEED, HEED, Polycomb protein EED, WAIT1, WAIT-1, WD protein associating with integrin cytoplasmic tails 1)

Cat no: E2211-50C

Supplier: United States Biological
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Polycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Jurkat cell lysate, 3T3/A31 cell lysate Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: E2211-50C
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: Western Blot
Size: 100ug
Form: Supplied a a liquid in Tris-Glycine, pH7.4, 150mM sodium chloride, 0.05% sodium azide.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. The Protein Data Bank, www.pdb.org, DOI 10.2210/pdb2qxv/pdb 2. Kuzmichev., A., et al. (2004). Mol Cell. 14(2):183-93. 3. Montgomery., ND., et al. (2005). Curr Biol. 15(10):942-7. 4. van der Vlag J, Otte AP (1999). Nat. Genet. 23 (4): 474
Additional info: Recognizes human EED. Species Crossreactivity: mouse. Species sequence homology: rat, opossum, chicken, dog, cow.

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