EED (Embryonic ectoderm development, hEED, HEED, Polycomb protein EED, WAIT1, WAIT-1, WD protein associating with integrin cytoplasmic tails 1)

Polycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nJurkat cell lysate, 3T3/A31 cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

E2211-50C

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied a a liquid in Tris-Glycine, pH7.4, 150mM sodium chloride, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. The Protein Data Bank, www.pdb.org, DOI 10.2210/pdb2qxv/pdb 2. Kuzmichev., A., et al. (2004). Mol Cell. 14(2):183-93. 3. Montgomery., ND., et al. (2005). Curr Biol. 15(10):942-7. 4. van der Vlag J, Otte AP (1999). Nat. Genet. 23 (4): 474

Additional Info

Recognizes human EED. Species Crossreactivity: mouse. Species sequence homology: rat, opossum, chicken, dog, cow.