Home  >  Products  >  EED (embryonic ectoderm development, hEED, HEED, Polycomb protein EED, WAIT1, WAIT-1, WD protein associating with integrin cytoplasmic tails 1)
EED (embryonic ectoderm development, hEED, HEED, Polycomb protein EED, WAIT1, WAIT-1, WD protein associating with integrin cytoplasmic tails 1)

EED (embryonic ectoderm development, hEED, HEED, Polycomb protein EED, WAIT1, WAIT-1, WD protein associating with integrin cytoplasmic tails 1)

Cat no: E2211-50D

Supplier: United States Biological
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
Read More on Supplier Website
Polycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:5000 Optimal dilutions to be determined by the researcher. Positive Control: A431 Cell Lysate Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: E2211-50D
Reactivities: Human, Mouse
Hosts: Mouse
Applications: Western Blot
Size: 100ug
Form: Supplied as a liquid in 0.1 M Tris-Glycine, pH7.4, 150mM sodium chloride, 0.05% sodium azide.
P type: Mab
Isotype: IgG2a,k
Purity: Purified by Protein G affinity chromatography.
References: 1. The Protein Data Bank, www.pdb.org, DOI 10.2210/pdb2qxv/pdb 2. Pasini, D., et al. (2007). Mol. Cell. Biol. 27, 3769-3779. 3. Bracken, A.P., et al. (2003). EMBO J. 22: 5323-5335. 4. Pasini, D., et al. (2004). 5. Bracken, A.P., et al. (2007). Cell Cycle. 3: 396-400 6. Bracken, A.P., et al. (2006). Genes Dev. 20: 1123-1136.
Additional info: Recognizes mouse EED (5-6 isoforms of EED are detected). Species Crossreactivity: human.

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
Enquiry_down_arrow
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*
See Full Product Listings >
Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave