EED, Recombinant, Human, aa1-441, His-Tag (Polycomb Protein EED Isoform A, Embryonic Ectoderm Development, HEED, WAIT1)

EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. \n\nSource: \nRecombinant protein corresponding to human EED, fused to His-tag at N-terminal expressed in E.coli.\n\nMolecular Weight: \n~52.6kD (464aa)\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MGSMSEREVS TAPAGTDMPA AKKQKLSSDE NSNPDLSGDE NDDAVSIESG TNTERPDTPT NTPNAPGRKS WGKGKWKSKK CKYSFKCVNS LKEDHNQPLF GVQFNWHSKE GDPLVFATVG SNRVTLYECH SQGEIRLLQS YVDADADENF YTCAWTYDSN TSHPLLAVAG SRGIIRIINP ITMQCIKHYV GHGNAINELK FHPRDPNLLL SVSKDHALRL WNIQTDTLVA IFGGVEGHRD EVLSADYDLL GEKIMSCGMD HSLKLWRINS KRMMNAIKES YDYNPNKTNR PFISQKIHFP DFSTRDIHRN YVDCVRWLGD LILSKSCENA IVCWKPGKME DDIDKIKPSE SNVTILGRFD YSQCDIWYMR FSMDFWQKML ALGNQVGKLY VWDLEVEDPH KAKCTTLTHH KCGAAIRQTS FSRDSSILIA VCDDASIWRW DRLR\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

137049

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 0.15M NaCl, 10% glycerol, 1mM DTT.

Purity

~85% (SDS-PAGE)

References

1. Rietzler M, Bittner M, Kolanus W, Schuster A, Holzmann B (Nov 1998). J Biol Chem 273 (42): 27459-66 2. Schumacher A, Lichtarge O, Schwartz S, Magnuson T (Jan 1999). Genomics 54 (1): 79-88.