Home  >  Products  >  eEF2k (Eucharyotic Elongation Factor-2 kinase, eEF-2 kinase)
eEF2k (Eucharyotic Elongation Factor-2 kinase, eEF-2 kinase)

eEF2k (Eucharyotic Elongation Factor-2 kinase, eEF-2 kinase)

Cat no: E2213-50A

Supplier: United States Biological
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
Read More on Supplier Website
Eukaryotic elongation factor-2 kinase (eEF-2k) previously known as +/calmodulin-dependent protein kinase III, is an abundant cytoplasmic protein highly specific for elongation factor-2 (eEf-2) (1). Phosphorylation of eEF-2 by eEF-2 kinase on specific threonine residues results in the inactivation of eEF-2 and in termination of mRNA translation 2). The activity of eEF2 kinase is not only dependent upon Ca2+ ions, calmodulin (CaM) and insulin, but is also regulated both negatively and positively by phosphorylation by different protein kinases (AMPK, S6K1, p90 RSK) (3-4). There is also evidence that eEF-2 phosphorylation is involved in the regulation of cell cycle progression, cellular differentiation, oogensis and malignant tumors (5-6). Applications: Suitable for use in Western Blot Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1: 5,000-50,000 Immunoprecipitation: 1:20 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Catalogue number: E2213-50A
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1. Ryazanov et al. Proc Nat Acad Sci 94: 4884-4889, 1997. 2. Ryazanov AG, et al. Nature 334:170-173, 1988. 3. Diggle, TA, et al. FEBS Lett 457:189-192, 1999. 4. Wang, XW, EMBO J 20:4370-4379, 2001. 5. End D, et al. J Biol Chem 257:9223-9225, 1988. 6. Severinov, KV, et al. New Biol 2: 887-893, 1990.
Additional info: Recognizes human eEF2k.

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
Enquiry_down_arrow
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*
See Full Product Listings >
Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave