eEF2k (Eucharyotic Elongation Factor-2 kinase, eEF-2 kinase)

Eukaryotic elongation factor-2 kinase (eEF-2k) previously known as +/calmodulin-dependent protein kinase III, is an abundant cytoplasmic protein highly specific for elongation factor-2 (eEf-2) (1). Phosphorylation of eEF-2 by eEF-2 kinase on specific threonine residues results in the inactivation of eEF-2 and in termination of mRNA translation 2). The activity of eEF2 kinase is not only dependent upon Ca2+ ions, calmodulin (CaM) and insulin, but is also regulated \nboth negatively and positively by phosphorylation by different protein kinases (AMPK, S6K1, p90 RSK) (3-4). There is also evidence that eEF-2 phosphorylation is involved in the regulation of cell cycle progression, cellular differentiation, oogensis and malignant tumors (5-6). \n\nApplications: \nSuitable for use in Western Blot Immunoprecipitation. Other applications not tested.\n \nRecommended Dilutions: \nWestern Blot: 1: 5,000-50,000 \nImmunoprecipitation: 1:20 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

E2213-50A

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Ryazanov et al. Proc Nat Acad Sci 94: 4884-4889, 1997. 2. Ryazanov AG, et al. Nature 334:170-173, 1988. 3. Diggle, TA, et al. FEBS Lett 457:189-192, 1999. 4. Wang, XW, EMBO J 20:4370-4379, 2001. \n5. End D, et al. J Biol Chem 257:9223-9225, 1988. 6. Severinov, KV, et al. New Biol 2: 887-893, 1990.

Additional Info

Recognizes human eEF2k.