EF1, alpha (Elongation Factor 1a)

Human eukaryotic translation elongation factor 1 alpha has two isoforms, alpha 1 and alpha 2. The alpha 1 isoform gene encodes a subunit of the elongation factor-1 complex which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. The alpha 1 isoform is expressed in brain, placenta, lung, liver, kidney, and pancreas. The other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. The alpha 1 isoform is identified as an autoantigen in 66% of patients with Felty syndrome. The gene for the alpha 1 isoform has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. The gene for the alpha 2 may be critical in the development of ovarian cancer.\n\nApplications:\nSuitable for use in Western Blot and Immunoprecipiation. Other applications not tested. \n\nRecommended Dilutions: \nWestern Blot: 0.5-2ug/ml detects EF-1a in RIPA lysates from non-stimulated A431 cells. Cell lysate was resolved by electrophoresis, transferred to nitrocellulose, and probed with E2212 (0.5ug/ml). Proteins were visualized using an IgG (HRP) Gt xMo and a chemiluminescence detection system.\nImmunoprecipitation: 4ug immunoprecipitated EF-1 a from an A431 RIPA cell lysate.\nOptimal dilution determined by the researcher. \n\nPositive Antigen Control: (Included)\nA0001-50: Non-stimulated A431 cell lysate.\n\nControl cells: Non-stimulated A431, Jurkat or 3T3/A31 cell lysates\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E2212

Size

200ug

Applications

IP, WB

Hosts

Mouse

Reactivities

Yeast/Fun

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Kimball SR, et al., (1999) J. Biol. Chem. 274:11647-11652. 2. Kaur K. and Ruben L. J., (1994) Biol Chem. 269:23045-23050. 3. Shang Y and Brown M., (2002) Science 295:2465-68. 4. Benkirane M. et al., (1998) J Biol Chem. 273:24898-905.

Additional Info

Recognizes EF-1a (Elongation factor-1a) at 53kD. 38kD protein occasionally detected in A431 cell lysate. Species Crossreactivity: Trypanosomes, yeast and mammals.