Effector Cell Protease Receptor-1 (EPR-1)

The inhibitors of apoptosis proteins (IAPs) are a widely expressed gene family of apoptotic inhibitors. Recently, a new human gene encoding a structurally and unique IAP designated Survivin has been identified. Survivin (human 142 aa, ~16.5kD) is highly expressed in less-differentiated embryonic cells or rapidly dividing tumor cell but not in fully differentiated adult tissues. Interestingly, Survivin coding strand has significant sequence homology with Effector cell Protease Receptor-1 (EPR-1) suggesting a potential for functional interaction between these two proteins.\nFactor Xa plays a critical role in the coagulation process by catalyzing the activation of prothrombin to thrombin. It acts on leukocytes, endothelium and smooth muscle cells triggering complex pathways of intracellular signaling. Factor Xa binds to EPR-1. The full length EPR-1 predicts a protein of 337 aa (~65kD de-glycosylated form). EPR-1 is expressed in vascular endothelial cells and smooth muscle cells. Survivin and EPR-1 are encoded by structurally and topographically distinct messages from potentially originating from gene cluster at chromosome 17q25. Overexpression of EPR-1 increased apoptosis and inhibited growth of transformed cells. The importance of the Survivin-EPR-1 interaction remains to be elucidated.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000 EPR-1 is ~62kD protein in detergent solubilized extracts of Jurkat and monocytic THP-1 cells.\nELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nE2212-53 Effector Cell Protease Receptor-1, Human Control Peptide\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E2212-50

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS,, 0.05% azide, before the addition of glycerol to 40%

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Ambrosini G et al (1997) Nature Med. 3:917-921. 2. Altieri DC et al (1994) J Biol. Chem. 269:3139-3142. 3. Altieri DC et al (1994) Biochem. 33:13848-13855. 4. Adida C et al (1998) Am. J Pathol. 152:43-49. 5. Lu C-D et al (1998) Cancer Res. 58:1808-1812. 6. Li F et al (1998) Nature 396:580-584.

Additional Info

Recognizes human Effector Cell Protease Receptor-1. Sequence homology: No homology with Survivin.