EFHD1 (EF-hand Domain-containing Protein D1, EF-hand Domain-containing Protein 1, Swiprosin-2, SWS2, PP3051)

EFHD1, also known as Swiprosin-2 or SWS2, is an EF-hand and coiled-coil-containing adaptor protein identified in a subtractive hybridization study using a neuronal cell line established from the cerebellum of an adult p53-null mouse, however further study indicated no difference in normal mice. Its mRNA is widely expressed, with its expression in brain undetectable at embryonic stages, with increasing levels from postnatal to adult development. In situ hybridization showed expression in neurons but not white matter of the cerebellum and cerebrum. EHFD1 is also highly expressed in testes, ovary, and the collecting ducts of the kidney, suggesting that in non-neuronal cells, EFHD1 may be involved in gametogenesis and water-reabsorbtion.\n\nApplications:\nSuitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 2-4ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman Spleen Tissue Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146618

Size

100ug

Applications

ELISA, IF, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Tominaga M and Tomooka Y. Novel genes cloned from a neuronal cell line newly established from a cerebellum of an adult p53-/- mouse. Biochem. Biophys. Res. Comm. 2002; 297:473-9.

Additional Info

Recognizes human EFHD1. Species Crossreactivity: mouse and rat.