EG5 (KIF11, Kinesin-like Protein KIF11, Kinesin-like Protein 1, Kinesin-like Spindle Protein HKSP, Kinesin-related Motor Protein Eg5, Thyroid Receptor-interacting Protein 5, TR-interacting Protein 5, TRIP-5, KNSL1, TRIP5)

Eg5 (also known as kinesin-like protein KIF11, kinesin-related motor protein Eg5, kinesin-like spindle protein HKSP, and thyroid receptor interacting protein 5 (TRIP5)) is a 119kD kinesin-like protein family, BimC subfamily. This protein is a catalytic kinesin motor with coiled-coil and Smc domains. Eg5 is localized at the centrosomes, spindle microtubules, and intracellular bridge. This motor protein is required for establishing the bipolar spindle. hEg5 is modified by phosphorylation on Thr927 by Cdc2 to allow association with the spindle apparatus. Eg5 has been shown to interact with the thyroid hormone receptor in presence of thyroid hormone and Cdc2.\n\nApplications:\nSuitable for use in Immunoprecipitation, Immunofluorescence and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E2213-95

Size

25ug

Applications

IF, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG1,k

References

1. Lee J, et al. 1995. Mol. Endocrinol. 9:243. 2. Blangy A, et al. 1995. Cell 83:1159. 3. Whitehead C, et al. 1998. J. Cell Sci. 111:2551. 4. DeBonis S, et al. 2003. Biochemistry. 42:338.

Additional Info

Recognizes human Eg5. Species Crossreactivity: mouse.