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EGFP (Enhanced Green Fluorescent Protein)

EGFP (Enhanced Green Fluorescent Protein)

Cat no: E2213-98

Supplier: United States Biological
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Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:5000 Immunohistochemistry: 1:1000-1:5000 Optimal dilutions to be determined by the researcher. Excitation max (nm): 488 Emission max (nm): 509 Extinction coefficient (Cm-1M-1): 61000 Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: E2213-98
Reactivities: Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ul
Form: Supplied as a lyophilized powder. Reconstitute in 100ul of sterile ddH2O. Centrifuge to remove any insoluble material.
P type: Pab
Isotype: IgG
Purity: Serum
References: 1. Chalfie, M., Tu, Y. et al (1994) Science 263:802-805. 2. Tsien, R. Y. (1998) Annu Rev Biochem 67:509-544. 3. Dardalhon, V. et al (1999) Hum Gene Ther 10:5-14. 4. Kain, S. R. (1999) DDT 4:304-312.
Additional info: Recognizes EGFP.

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