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EGFR, Phosphorylated (Tyr1016) (Epidermal Growth Factor Receptor, Proto-oncogene c-ErbB-1, Receptor Tyrosine-protein Kinase erbB-1, ERBB1)

EGFR, Phosphorylated (Tyr1016) (Epidermal Growth Factor Receptor, Proto-oncogene c-ErbB-1, Receptor Tyrosine-protein Kinase erbB-1, ERBB1)

Cat no: E3375-03L

Supplier: United States Biological
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The EGFR, epidermal growth factor receptor, belongs to a family of receptor tyrosine kinases in mammals which is composed of four members: EGFR (ErB1), ErB2, ErB3, and ErB4. EGFR is a 1210aa residue trans-membrane glycoprotein. It consists of an extracellular ligand binding domain, a single a-helical transmembrane pass, an intracellular tyrosine kinase domain, and a COOH-terminal region that contains autophosphorylation sites. The binding of specific polypeptide ligands, including EGF, transforming growth factor-a, betacellulin, heparin-binding EGF, epiregulin, or amphiregulin, results in phosphorylation of multiple tyrosine residues in the COOH-terminal tail, such as tyrosine 869Y, 1016Y, 1092Y, 1110Y, 1125Y, 1172Y, 1197Y, etc. The binding of phosphorylated EGFR tyrosines with downstream signaling proteins initiates multiple signaling cascades that culminate in cell proliferation, migration, protein secretion, differentiation and/or oncogenesis. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: E3375-03L
Reactivities: Human
Hosts: Rabbit
Applications: Western Blot
Size: 25ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Wells, A. et al. Int J Biochem Cell Biol 31, 637 (1999). 2. Ullrich, A. et al. Cell 61, 203 (1990). 3. Ullrich, A. et al. Nature 309, 418 (1984).
Additional info: Recognizes human EGFR when phosphorylated at Tyr1016. Species sequence homology: mouse and rat.

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