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Egr-2b, ID (Early Growth Response 2b, egr2b, Dr.75080)

Cat no: E2215-33A

Egr-2b, ID (Early Growth Response 2b, egr2b, Dr.75080)

Early growth response 2b (egr2b) is also known as krox20. First detected during late gastrulation, found in the prospective neuroepithelium domain around 100% epiboly and begins to constrict along the neuroaxis by the end of gastrulation - around 10 hours post- fertilization (hpf). By 12hpf expression is restricted in two regions of the neuroepithelium of the prospective hindbrain; rhombomeres 3 and 5. Around 14hpf neural crest migration begins from the dorsal surface of R5 moving caudally into R6 and then ventrally towards the pharyngeal arches. Crest migration is not apparent at or after 16hpf and no migration is seen from R3. Expression is down-regulated first in R3 around 26hpf and later in R5 around 30hpf. Egr2b binds two specific DNA sites located in the promoter region of HOXA4.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:5000-1:20,000\nWestern Blot: 1:500-1:1000\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: E2215-33Q\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E2215-33A

Size

250ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

ZF/Fish

Form

Supplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Oxtoby, E et al (1993) Nucleic Acids Res. 21:1087-1095. 2. http://www.uniprot.org/uniprot/Q05159.

Additional Info

Recognizes zebrafish Egr-2b.

Read more on Supplier website

Applications

FC

Hosts

Mouse

Reactivities

Hum

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Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info
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