Early growth response 2b (egr2b) is also known as krox20. First detected during late gastrulation, found in the prospective neuroepithelium domain around 100% epiboly and begins to constrict along the neuroaxis by the end of gastrulation - around 10 hours post- fertilization (hpf). By 12hpf expression is restricted in two regions of the neuroepithelium of the prospective hindbrain; rhombomeres 3 and 5. Around 14hpf neural crest migration begins from the dorsal surface of R5 moving caudally into R6 and then ventrally towards the pharyngeal arches. Crest migration is not apparent at or after 16hpf and no migration is seen from R3. Expression is down-regulated first in R3 around 26hpf and later in R5 around 30hpf. Egr2b binds two specific DNA sites located in the promoter region of HOXA4.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:5000-1:20,000
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.
Control Peptide: E2215-33Q
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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