EHD1, or EH-domain containing 1, belongs to a highly conserved family of EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. EHD1 is thought to play a role in the endocytosis of IGF1 receptors (1).
Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:1000-10,000
Immunohistochemistry (Paraffin): 1:100-250
Immunocytochemistry: 1:100-250
Immunoprecipitation: 1:10-100
Optimal dilutions to be determined by the researcher.
Positive Control:
Western Blot: HepG2, human testis, SW480, SK-OV-3, and HeLa lysates
Immunohistochemistry: Human kidney tissue, human tonsil tissue
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.