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eIF-2alpha (EIF2S1, EIF2, EIF-2, EIF2A, eIF-2A, EIF-2A, EIF-2alpha, eIF-2-alpha, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, Eukaryotic translation initiation factor 2 subunit 1 alpha 35k

Cat no: E9108-48B

eIF-2alpha (EIF2S1, EIF2, EIF-2, EIF2A, eIF-2A, EIF-2A, EIF-2alpha, eIF-2-alpha, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, Eukaryotic translation initiation factor 2 subunit 1 alpha 35k

Phosphorylation of the alpha subunit of eukaryotic initiation factor 2 is a well documented mechanism of downregulating protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers the Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). For eIF2 to promote a new round of translation initiation, GDP must be exchanged for GTP, a reaction catalyzed by eIF2B (1,2). Kinases activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2) and hemin deficiency (HRI) can phosphorylate the alpha subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2- GDP-eIF2B complex, inhibiting the turnover of eIF2B. Induction of PKR by IFN-gamma and TNF-alpha, or stress provoked by depletion of endoplasmic reticulum calcium levels, induces potent phosphorylation of eIF2alpha at Ser51 (5,6).\n\nApplications: \nSuitable for use in Western Blot. Other applications have not been tested. \n\nRecommended Dilution: \nWestern Blot: 1:1000 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E9108-48B

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

P Type

Pab

Purity

Purified by Protein A and peptide affinity chromatography

Isotype

IgG

References

(1) Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25

Additional Info

Detects endogenous levels of total eIF-2 alpha protein. Recognizes human, mouse, monkey and rat eIF-2alpha .

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