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eIF-6 (EIF6, EIF3A, Eukaryotic Translation Initiation Factor 6, B4 integrin interactor)

Cat no: E8949-37U

eIF-6 (EIF6, EIF3A, Eukaryotic Translation Initiation Factor 6, B4 integrin interactor)

Hemidesmosomes are structures that link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. eIF-6 binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. It is insoluble and found in both the nucleus and cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry and Flow cytometry. Other applications have not been tested. \n\nRecommended Dilutions: \nWestern Blot: 1:1000-1:10,000 \nImmunohistochemistry: 1:100-1:250\nFlow cytometry: 1:100-1:500\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

E8949-37U

Size

100ul

Applications

FC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.05% BSA, 0.01% sodium azide, 50% glycerol.

P Type

Mab

Purity

Supernatant

Additional Info

Recognizes human eIF-6. Species crossreactivity: Mouse, rat.

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