EIF2AK1 (HRI, KIAA1369, HCR, Heme-controlled repressor, Heme-regulated inhibitor, Hemin-sensitive initiation factor 2-alpha kinase, Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase)

EIF2AK1 (Eukaryotic Translation Initiation Factor 2-Alpha Kinase 1), also called HRI, is an enzyme that in humans is encoded by the EIF2AK1 gene. Hartz (2010) mapped the EIF2AK1 gene to chromosome 7p22.1 based on an alignment of the EIF2AK1 sequence with the genomic sequence. Chen and London (1995) reviewed the activation and control of rat and rabbit Hri. Hri functions as a dimer, and its activation involves autophosphorylation, which requires an invariant lysine in kinase domain II. Using cDNA arrays and Northern blot analysis, Hwang et al. (2000) found that HRI was downregulated in the majority of ovarian cancers examined compared with normal ovarian tissues.\n\nUniProt Number:\nQ9BQI3\n\nGene ID:\nEIF2AK1\n\nApplications:\nSuitable for use in Western Blot and Immunocytochemistry.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144622

Size

100ug

Applications

ICC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Chen, J.-J., London, I. M. Regulation of protein synthesis by heme-regulated eIF-2-alpha kinase. Trends Biol. 20: 105-108, 1995. \n2. Hartz, P. A. Personal Communication. Baltimore, Md. 10/25/2010.\n3. Hwang, S.-Y., Kim, M.-K., Kim, J.-C. Cloning of hHRI, human heme-regulated eukaryotic initiation factor 2-alpha kinase: down-regulated in epithelial ovarian cancers. Molec. Cells 10: 584-591, 2000.\n

Additional Info

Recognizes human h.EIF2AK1. No crossreactivity with other proteins.