EIF2AK3 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 3, PRKR-like Endoplasmic Reticulum Kinase, Pancreatic eIF2-alpha Kinase, HsPEK, PEK, PERK)

The PKR-like endoplasmic reticulum kinase (PERK, also know as Eukaryotic translation initiation factor 2-a kinase 3) is a type I transmembrane protein localized to the endoplasmic reticulum (ER). PERK consists of an N-terminal ER luminal domain, a membrane-spanning region, and a cytosolic C-terminal serine/threonine kinase domain. The luminal domain of PERK is bound to the ER chaperone GRP78 in unstressed cells. PERK activation occurs upon accumulation of misfolded proteins and the ER lumen, which triggers GRP78 dissociation from PERK thereby allowing PERK dimerization and autophosphorylation. PERK phosphorylates two established targets: the eukaryotic translation initiation factor 2-a(eIF2?, and the Nrf2 transcription factor. Phosphorylation of eIF2? results in attenuation of translation initiation. The translational block also contributes to cell cycle arrest due to loss of the G1 regulatory protein, cyclin D1. PERK-dependent phosphorylation of Nrf2 promotes transcription of phase II detoxifying enzymes which is critically important for elimination of intracellular reactive oxygen species. Thus, while inhibiting new protein synthesis and thereby decreasing the ER protein load PERK simultaneously induces expression of genes that help restore cellular redox homeostasis and promote survival.\n\nApplications:\nSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:4000-1:20,000\nWestern Blot: 1:500-1:3000\nImmunoprecipitation: 10-30ul\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts