eIF2C1 (Eukaryotic Translation Initiation Factor 2C1, eIF2C 1, eIF-2C 1, Argonaute 1, AGO1, hAgo1, Golgi Endoplasmic Reticulum Protein 95kD, GERP95, Protein Argonaute-1, Putative RNA-binding Protein Q99)

Small non-coding RNAs are important regulators of gene expression in higher eukaryotes (1,2). Several classes of small RNAs, including short interfering RNAs (siRNAs) (3), microRNAs (miRNAs) (4) and Piwi-interacting RNAs (piRNAs) (5), have been identified. MicroRNAs are\nabout 21 nucleotides in length and have been implicated in many cellular processes such as development, differentiation and stress response (1,2). MicroRNAs regulate gene expression by modulating mRNA translation or stability (2). MicroRNAs function together with the protein components in the complexes called micro-ribonucleoproteins (miRNPs) (2). Among the most important components in these complexes are Argonaute proteins (1,2). There are four members in the mammalian Argonaute family and only Argonaute 2 (Ago2) possesses the Slicer endonuclease activity (1,2). Argonaute proteins participate in the various steps of\nmicroRNA-mediated gene silencing such as repression of translation and mRNA turnover (1).\n \nApplications: \nSuitable for use in RIA, Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunohistochemistry (Paraffin): 1:1000\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E2237-27

Size

100ul

Applications

ELISA, FC, ICC, IF, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Purified

Isotype

IgG

References

(1) Peters, L. and Meister, G. (2007) Mol Cell 26, 611-23. (2) Filipowicz, W. et al. (2008) Nat Rev Genet 9, 102-14. (3) Caplen, N.J. et al. (2001) Proc Natl Acad Sci USA 98, 9742-7. (4) Mourelatos, Z. et al. (2002) Genes Dev 16, 720-8. (5) Girard, A. et al. (2006) Nature 442, 199-202.

Additional Info

Recognizes endogenous levels of total Argonaute 1 protein. Species Crossreactivity: monkey, mouse, rat.

Alternative Names

Eukaryotic Translation Initiation Factor 2C, 1, DKFZp686M13167