EIF2S1 (Eukaryotic Translation Initiation Factor 2, Subunit 1 alpha, eIF2 alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, EIF2A, Eukaryotic Translation Initiation Factor 2 Subunit 1)

EIF-2alpha, also known as eukaryotic translation initiation factor 2alpha, promotes the binding of the initiator tRNA to the 40S ribosomal subunits and Positive Controls translational rates via phosphorylation/dephosphorylation mechanisms. The double-stranded RNA (dsRNA)-activated protein kinase (PKR) provides an important Positive Control in the regulation of protein synthesis initiation through phosphorylation of eIF-2alpha. Phosphorylated eIF-2 alpha inhibits cell growth and induces apoptosis. Phosphorylation of eIF-2alpha occurs under a variety of conditions including viral infection, apoptosis, nutrient deprivation, heme-deprivation, and certain stresses. eIF-2 exists as a heterotrimeric protein composed of 3 nonidentical subunits, alpha (36kD), beta (38kD), and gamma (52kD).\n\nApplications:\nSuitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nAA Sequence:\nMPGLSCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSRRRIRSINKLIRIGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRHVAEVLEYTKDEQLESLFQRTAWVFDDKYKRPGYGAYDAFKHAVSDPSILDSLDLNEDEREVLINNINRRLTPQAVKIRADIEVACYGYEGIDAVKEALRAGLNCSTENMPIKINLIAPPRYVMTTTTLERTEGLSVLSQAMAVIKEKIEEKRGVFNVQMEPKVVTDTDETELARQMERLERENAEVDGDDDAEEMEAKAED\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

126230

Size

100ug

Applications

ELISA, IF, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1,k

References

1. IGF2BP1 enhances HCV IRES-mediated translation initiation via the 3'UTR. Weinlich S, Huttelmaier S, Schierhorn A, Behrens SE, Ostareck-Lederer A, Ostareck DH.RNA. 2009 Aug;15(8):1528-42. Epub 2009 Jun 18.

Additional Info

Recognizes human EIF2S1.