EIF3B is an acidic protein belonging to the EIF-3 subunit B family with an RRM (RNA recognition motif) domain. EIF3B is involved in protein translation:synthesis where it binds to the 40S ribosomal subunit and promotes the binding of methionyl-tRNAi and mRNA. EIF3B is a component of the EIF3 multiprotein complex, composed of at least 13 different subunits. It is also known to interact with EIF3A and directly with the p170 subunit of eIF3, and the region required for this association is located within the RRM. EIF3B is ubiquitously expressed in most of the tissues.
Applications:
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.