eIF3G (Eukaryotic Translation Initiation Factor 3 Subunit G, eIF3g, Eukaryotic Translation Initiation Factor 3 RNA-binding Subunit, eIF-3 RNA-binding Subunit, Eukaryotic Translation Initiation Factor 3 Subunit 4, eIF-3-delta, eIF3 p42, eIF3 p44, EIF3S4)

eIF3G is an RNA-associated protein involved in protein synthesis. It is a member of the eIF-3 subunit G family and the largest of the eukaryotic translation initiation factor family with eukaryotic translation initiation factor-3 (eIF3) being a multiprotein complex composed of at least 13 different subunits. eIF3G has a RRM (RNA recognition motif) domain and is known to interact with eIF3K. The function of eIF3G is to bind to the 40S ribosomal subunit and promote the binding of methionyl-tRNAi and mRNA. This subunit of eukaryotic translation initiation factor-3 also binds to the 18S rRNA. It is ubiquitously expressed in most tissues.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.025-0.1ug/ml\nImmunohistochemistry (formalin fixed paraffin embedded): 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E8949-36E

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Can

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Block, KL. et al. J. Biol. Chem. 273:31901-31908 (1998). 2. Bandyopadhyay, A. et al. Nucleic Acids Res. 27:1331-1337 (1999).

Additional Info

Recognizes human eIF3G. Species Crossreactivity: chimpanzee, bovine, canine, equine, mouse, rat, rhesus monkey. Species sequence homology: xenops, zebrafish, chicken.