Home  >  Products  >  eIF3J (Eukaryotic Translation Initiation Factor 3 Subunit J, eIF3j, Eukaryotic Translation Initiation Factor 3 Subunit 1, eIF-3-alpha, eIF3 p35, EIF3S1, PRO0391)
eIF3J (Eukaryotic Translation Initiation Factor 3 Subunit J, eIF3j, Eukaryotic Translation Initiation Factor 3 Subunit 1, eIF-3-alpha, eIF3 p35, EIF3S1, PRO0391)

eIF3J (Eukaryotic Translation Initiation Factor 3 Subunit J, eIF3j, Eukaryotic Translation Initiation Factor 3 Subunit 1, eIF-3-alpha, eIF3 p35, EIF3S1, PRO0391)

Cat no: E8949-34B

Supplier: United States Biological
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eIF3J is an RNA-associated protein belonging to the eukaryotic translation initiation factor family. It belongs to the group of proteins involved in protein translation:synthesis where it binds to the 40S ribosomal subunit and promotes the binding of methionyl-tRNAi and mRNA to form the 40S initiation complex. eIF3J is a component of the eIF3 multiprotein complex, composed of at least 13 different subunits. It is also known to interact with eIF3S12. Ubiquitous expression is seen in most tissues. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Positive Control: 293 or 3T3 cell lysate Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: E8949-34B
Reactivities: Human, Mouse, Canine
Hosts: Rabbit
Applications: Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Block, KL. et al. J. Biol. Chem. 273:31901-31908 (1998). 2. Fraser, CS. et al. Molec. Cell. 26:811-819 (2007). 3. Fraser, CS. et al. J. Biol. Chem. 279:8946-8956 (2004).
Additional info: Recognizes human eIF3J. Species Crossreactivity: chimpanzee, canine, equine, mouse, orangutan. Species sequence homology: bovine, zebrafish, chicken.

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