EIF4A1 (Eukaryotic Initiation Factor 4A-I, eIF-4A-I, eIF4A-I, ATP-dependent RNA Helicase eIF4A-1, DDX2A, EIF4A)

EIF4A1 is a 406aa ATP-dependent RNA helicase belonging to the DEAD box helicase family and eukaryotic initiation factor-4A subfamily with a helicase ATP-binding domain and a helicase C-terminal domain. EIF4F eukaryotic translation initiation complex is formed by the association of eIF4A1 with eIF4E and eIF4E kinase (MNK1), eIF4B, eIF4G, and PABPC1. It is closely related to eIF4A2. EIF4A1 is a eukaryotic translation initiation factor involved in protein translation:synthesis where it helps in unwinding the secondary structure of RNA in the 5'-UTR of mRNAs, cap recognition, and is necessary for efficient binding of mRNA to the 40S small ribosomal subunit, as well as subsequent scanning for the initiator codon. Ubiquitous expression of the enzyme eIF4A1 is seen in most tissues.\n\nApplications:\nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:100-1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

E8949-39N

Size

100ug

Applications

ELISA

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Moll

Form

Supplied as a liquid, 0.025% sodium azide, 50% glycerol.

P Type

Pab

Purity

Serum.

Isotype

IgG

References

1. Jones, E. et al. Genomics. 53:248-250 (1998). 2. Kukimoto, I. et al. Biochem. Biophys. Res. Commun. 233:844-847 (1997). 3. Nielsen, PJ. et al. EMBO J. 7:2097-2105 (1988).

Additional Info

Recognizes human eIF4A1. Species Crossreactivity: chimpanzee, macaque, mouse, orangutan, rat, rhesus monkey. Species sequence homology: canine, opossum.